Colloid &  Nanoscience  Journal

Colloid & Nanoscience Journal

Using green-synthesized nanoparticles in the preparation of polyvinyl chloride-based nanocomposite membranes with antibacterial activity in aqueous solutions

Document Type : Original Article

Authors
1 Department of Chemistry, Faculty of Science, Arak University, Arak , Iran
2 Department of Biology, Faculty of Science, Arak University, Arak 38156-8-8349, Iran
Abstract
Metal nanoparticles are generally prepared through chemical and physical reactions, which are mostly expensive. There are many concerns about environmental and biological contamination caused by current nanoparticle production conditions in the long term. In response to this challenge, today, the approach of some research is directed towards the synthesis of nanoparticles using biological systems (biological method). In these methods, the ionic state of the metal is used to produce nanoparticles. Lower cost, reduced production of pollutants, and maintaining environmental safety for human health are the advantages of green synthesis over conventional nanoparticle synthesis methods. In this research, silver nanoparticles(AgNPs) produced by Pseudomonas bacteria were used to prepare antibacterial membranes. To investigate the antibacterial activity of the membranes, 0.25, 0.50 and 1.00 wt.% of these nanoparticles were used in the mixture of membranes prepared by the phase inversion method. The structure of the membranes was investigated using X-ray fluorescence spectroscopy. The DLS analyzer determined the size of the synthesized nanoparticles under optimal conditions to be in the nanometer range. valuate the antibacterial properties of the membranes, the disk test and the bacteria Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were used. To investigate the performance of the membranes, their flux and their rejection were tested. The results showed that the permeability and antibacterial properties of the membranes against gram-positive and gram-negative bacteria increased clearly with the increase of nanoparticles prepared by the green synthesis method.

Graphical Abstract

Using green-synthesized nanoparticles in the preparation of polyvinyl chloride-based nanocomposite membranes with antibacterial activity in aqueous solutions
Keywords

[1] D. Seckler, R. Barker, U. Amarasinghe, Water scarcity in the twenty-first century, Int. J. Water Resour. Dev. 15 (1999) 29–42. doi.org/10.1080/07900629948916
 
[2] R. W. Baker, "Membrane Technology and Applications", Third Edition, John Wiley & Sons Ltd, California, 2012.
[3] M. Mousavi, M. Soltanieh, Appllication of charged membranes in water softening: modeling and experiments in the presence of polyelectrolyte", J. Membr. Sci, 154 (1999), 53-60. doi.org/10.1016/S0376-7388(98)00285-3
[4] M. Mukherjee, S. De, Antibacterial polymeric membranes: a short review, Environ Sci, 4 (2018), 1078-1104. doi:10.1039/C8EW00206A
[5] P.T. P. Aryanti, M. Sianipar, M. Zunita and Wenten I.G., Modified membrane with antibacterial properties, Membr. Water Treat., 8 (2017), 463-481. DOI:10.12989/mwt.2017.8.5.463
[6] G. D. Kang, C. J. Gao, W. D. Chen, X. M. Jie, Y. M. Cao, Q. Yuan, Study on hypochlorite degradation of aromatic polyamide reverse osmosis membrane, J. Membr. Sci, 300 (2007), 165-171. doi.org/10.1016/j.memsci.2007.05.025
[7] N. P. Soice, A. C. Maladono, D. Y. Takigawa, A. D. Norman, W. B. Krantz, A. R. Greenberg, Oxidative degradation of polyamide reverse osmosis membranes: studies of molecular model compounds and elected membranes, J. Appl. Polym. Sci., 90 (2003), 1173–1184. doi.org/10.1002/app.12774
[8] H. C. Flemming, G. Schaule, R. Mcdonogh, Biofouling on Membranes, A Short Review, Exp. Therm. Fluid Sci., 14 (1997), 382-391. DOI:10.1007/978-94-011-1824-8-43
[9] J. Zhu, J. Hou, Y. Zhang, M. Tian, T. He, J. Liu, V. Chen, Polymeric antimicrobial membranes enabled by nanomaterials for water treatment, J. Membr. Sci., 550 (2018), 173–197. doi.org/10.1016/j.memsci.2017.12.071
[10] X. Chen, H. J. Schluesener, Nano-silver: a nanoproduct in medical application, Toxicol. Lett., 176 (2008), 1–12. doi.org/10.1016/j.toxlet.2007.10.004
[11] A. Nanda, M. Saravanan, Biosynthesis of silver nanoparticles from Staphylococcus aureus and its antimicrobial activity against MRSA and MRSE, Nanomed.: Nanotechnol. Biol. Med., 5 (2009), 452–456. doi.org/10.1016/j.nano.2009.01.012
[12] S. L. Percival, P. G. Bowler, J. Dolman, "Antimicrobial activity of silver-containing dressings on wound microorganisms using an in vitro biofilm model." Int. Wound J., 4 (2007) 186–191. doi.org/10.1111/j.1742-481X.2007.00296.x
 
[13] M. S. Yuksel, B. Tas, D .Y. Koseoglu-Imer, I. Koyuncu, Effect of silver nanoparticle (AgNP) location in nanocomposite membrane matrix fabricated with different polymer type on antibacterial mechanism, Desalination, 347 (2014) 120–130. doi.org/10.1016/j.desal.2014.05.022
[14] K. Vimala, Y. Murali Mohan, K. Samba Sivudu, K. Varaprasad, S. Ravindra, N. Narayana Reddy, Y. Padma, B. Sreedhar, K. MohanaRaju, Fabrication of porous chitosan films impregnated with silver nanoparticles: A facile approach for superior antibacterial application, Colloids and Surfaces B: Biointerfaces, 76 (2010), 248–258. doi.org/10.1016/j.colsurfb.2009.10.044
[15] H. Basri, A.F. Ismail, M. Aziz, Polyethersulfone (PES)–silver composite UF membrane: Effect of silver loading and PVP molecular weight on membrane morphology and antibacterial activity, Desalination, 273 (2011), 72–80. doi.org/10.1016/j.desal.2010.11.010
[16] C. Liu, A. Fonseca de Faria, J. Ma, M. Elimelech, Mitigation of biofilm development on thin-film composite membranes functionalized with zwitterionic polymers and silver nanoparticles, Environ. Sci. Technol., 54 (2017), 182-191. doi.org/10.1021/acs.est.6b03795
[17] Z. Yang, R. Takagi, X. Zhang, T. Yasui, L. Zhang, H. Matsuyama, Engineering a dual-functional sulfonated polyelectrolyte-silver nanoparticle complex on a polyamide reverse osmosis membrane for robust biofouling mitigation, J. Membr. Sci, 618 (2021), 118757. doi.org/10.1016/j.memsci.2020.118757
[18] S. M. Hosseini, S. S. Madaeni, A. R. Heidari, A. Amirimehr, "Preparation and characterization of ion-selective polyvinyl chloride based heterogencous cation exchange membrane modifted by magnetic iron-nickel oxide nanoparticles, Desalination, 284 (2012), 191-199. doi.org/10.1080/01496395.2010.534122
[19] M. Peyravi, A. Rahimpour, M. Jahanshahi, A. Javadi, and A. Shockravi, Tailoring the surface properties of PES ultrafiltration membranes to reduce the fouling resistance using synthesized hydrophilic copolymer, Microporous Mesoporous Mater., 160 (2012) 114–125. doi.org/10.1016/j.micromeso.2012.04.036
[20] Z. Yang, H. Guo, Z. K. Yao, Y. Mei, C. Y. Tang, Hydrophilic silver nanoparticles induce selective nanochannels in thin film nanocomposite polyamide membranes, Environ. Sci. Technol., 53 (2019), 5301−5308. DOI: 10.1021/acs.est.9b00473
[21] A. Behboudi, Y. Jafarzadeh and R. Yegani, Enhancement of antifouling and antibacterial properties of PVC hollow fiber ultrafiltration membranes using pristine and modified silver nanoparticles, J. Environ. Chem. Eng., 6 (2018), 1764−1773. doi.org/10.1016/j.jece.2018.02.031
[22] N. Haghighat, V. Vatanpour, M. Sheydaei, Z. Nikjavan, Preparation of a Novel Polyvinyl Chloride (PVC) Ultrafiltration Membrane Modified with Ag/TiO2 Nanoparticle with Enhanced Hydrophilicity and Antibacterial Activities, Sep. Purif. Technol., 237 (2020), 116374. doi.org/10.1016/j.seppur.2019.116374
[23] E. S. Mansor, A. M. Amer, S. S. Abelsalam, A. Radwan, M. O. Abdel-Monem, Silver Oxide Nanoparticles-Modified Poly Vinyl Chloride Membranes to Enhance the Antibacterial Properties, Egypt. Acad. J. Biol. Sci., 2022, Vol. 14, pp. 193- 204. doi.org/10.21608/EAJBSG.2022.274740
[24] N. Asmaa, Al-Himeiry, H. Alaa, Al-Fatlawi, The antibacterial activity of poly(vinyl chloride) membrane impregnated with silver nanoparticles, Mater. Today: Proc., 61 (2022), 706−709. doi.org/10.1016/j.matpr.2021.08.277
[25] A. Mollahosseini, A. Rahimpour, M. Jahamshahi, M. Peyravi, M. Khavarpour, The effect of silver nanoparticle size on performance and antibacteriality of polysulfone ultrafiltration membrane, Desalination, 306 (2012), 41-50. doi.org/10.3390/nano12030388
[26] M. A. M. Abo-State, A. M. Partila, Microbial production of silver nanoparticles by pseudomonas aeruginosa cell free extract, Journal of Ecology of Health & Environment, 3(2015), 91-98. doi.org/10.12785/jehe/030306
[27] M.H. El-Rafie, M.E. El-Naggar, M. A. Ramadan, Moustafa M.G. Foudaa, Salem S. Al-Deyab, A. Hebeish, Environmental synthesis of silver nanoparticles using hydroxypropyl starch and their characterization, Carbohydr. Polym. 86(2011), 630-635. doi.org/10.1016/j.carbpol.2011.04.088
Pseudomonas aeruginosa, Indian J. Biotechnol, 11 (2012), 72-76. doi.org/10.1016/j.colsurfb.2011.01.042
[28] D. Paul, S. Narayan Sinha, Extracellular synthesis of silver nanoparticles using pseudomonas aeruginosa KUPSB12 and its antibacterial activity, Jordan J. Biol. Sci., 7 (2014), 245 – 250. doi.org/10.12816/0008246
[29] C. G. Kumar, S. K. Mamidyala, Extracellular synthesis of silver nanoparticles using culture supernatant of Pseudomonas aeruginosa, Colloids Surf. B: Biointerfaces., 84 (2011), 462–466. DOI: 10.1016/j.colsurfb.2011.01.042
[30] M. Q. Khan, D. Kharaghann, N. Nishat, S. A. Shahzad, T. Hussain, K. OhKim, I. S. Kim, The fabrications and characterizations of antibacterial PVA/Cu nanofibers composite membranes by synthesis of Cu nanoparticles from solution reduction, nanofibers reduction and immersion methods, Mater. Res. Express, 6 (2019), 1591-2053. DOI 10.1088/2053-1591/ab1688
[31] X. Zhu, R. Bai, K. H. Wee, C. Liu, S. L. Tang, Membrane surfaces immobilized with ionic or reduced silver and their anti-biofouling performances, J. Membr. Sci., 363 (2010), 278–286. doi.org/10.1016/j.memsci.2010.07.041
[32] L. Shen, X. Bian, X. Lu, L. Shi, Z. Liu, L. Chen, Z. Hou, K. Fan, Preparation and characterization of ZnO/polyethersulfone (PES) hybrid membranes, Desalination, 293 (2012), 21-29. doi.org/10.1016/j.desal.2012.02.019
[33] E. Bagheripour, A. Moghadassi, S. Hosseini, Novel nanofiltration membrane with low concentration of polyvinylchloride: Investigation of solvents’ mixing ratio effect (Dimethyl acetamide/Tetrahydrofuran), Arab. J. Chem., 10 (2014), 3375-3380. doi.org/10.1016/j.arabjc.2014.01.019
[34] R. K. Matharu, L. Ciric, M. Edirisinghe, Nanocomposites: suitable alternatives as antimicrobial agents. Nanotechnology, 29 (2018), 282001. DOI 10.1088/1361-6528/aabbff
[35] L. P. Singh, S. K. Bhattacharyya, S. Ahalawat, R. Kumar, G. Mishra, U. Sharma, G. Singh, S. Ahalawat, Sol-Gel processing of silica nanoparticles and their applications, Adv Colloid Interface Sci., 214(2014), 17-37. doi: 10.1016/j.cis.2014.10.007
[36] L. George, S. B. Gudennavar, D. Joseph, S.G. Bubbly, KShell X-ray intensity ratios and vacancy transfer probabilities of Fe, Ag and Te following electron capture decay, Canadian Journal of Physics, 92 (2014), 1489-1493. doi.org/10.1139/cjp-2014-0105
[37] Gram Positive vs Gram Negative, https://www.technologynetworks.com/ Last updated: December 18, 2023 by Karen Steward.
[38] M. Rai, A. Yadav, A. Gade, Silver nanoparticles as a new generation of antimicrobials, Biotechnol Adv., 27 (2009), 76–83. doi.org/10.1016/j.biotechadv.2008.09.002
 
Volume 4, Issue 2
In Press
Summer 2026

  • Receive Date 14 November 2025
  • Revise Date 24 February 2026
  • Accept Date 28 January 2026
  • Publish Date 01 July 2026