Metal nanoparticles are generally prepared through chemical and physical reactions, which are mostly expensive. There are many concerns about environmental and biological contamination caused by current nanoparticle production conditions in the long term. In response to this challenge, today, the approach of some research is directed towards the synthesis of nanoparticles using biological systems (biological method). In these methods, the ionic state of the metal is used to produce nanoparticles. Lower cost, reduced production of pollutants, and maintaining environmental safety for human health are the advantages of green synthesis over conventional nanoparticle synthesis methods. In this research, silver nanoparticles(AgNPs) produced by Pseudomonas bacteria were used to prepare antibacterial membranes. To investigate the antibacterial activity of the membranes, 0.25, 0.50 and 1.00 wt.% of these nanoparticles were used in the mixture of membranes prepared by the phase inversion method. The structure of the membranes was investigated using X-ray fluorescence spectroscopy. The DLS analyzer determined the size of the synthesized nanoparticles under optimal conditions to be in the nanometer range. valuate the antibacterial properties of the membranes, the disk test and the bacteria Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were used. To investigate the performance of the membranes, their flux and their rejection were tested. The results showed that the permeability and antibacterial properties of the membranes against gram-positive and gram-negative bacteria increased clearly with the increase of nanoparticles prepared by the green synthesis method.