Synthesis of ternary nickel-cobalt-palladium nanoparticle by microemulsion method and investigation of its antibacterial activity on Escherichia coli

Namakchi, Bina; Saydi, Hassan

doi:10.61186/CNJ.2.3.390

Synthesis of ternary nickel-cobalt-palladium nanoparticle by microemulsion method and investigation of its antibacterial activity on Escherichia coli

Document Type : Original Article

Authors

Bina Namakchi

Hassan Saydi

Department of Chemistry, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran

10.61186/CNJ.2.3.390

Abstract

In this research, nickel-cobalt-palladium nanoparticles were synthesized using the microemulsion method. The formation of nanoparticles, morphology, size and size distribution of nanoparticles was investigated using SEM, UV-Vis, and DLS methods. The UV-visible spectrophotometric results showed a peak in the region of 380-480 nm, confirming the presence of nanoparticles. The SEM results indicated spherical particle sizes in the range of 45-50 nm for the ratio of Ni:Co:Pd (1:1:1). Additionally, the DLS results showed a size range of produced nanoparticles between 50 and 100 nm, which is consistent with the SEM and UV-Visible results. Nickel-cobalt-palladium nanoparticles exhibited antibacterial properties against E. coli bacteria. The diameter of the zone of inhibition formed in samples containing nanoparticles was significantly larger than the diameter of the positive control sample and the negative control sample (without a zone of inhibition), thus confirming their antibacterial properties.

Keywords

Nanoparticles

Antibacterial properties

Escherichia coli

Microemulsion

[1] L. Zhang, Y. Jiang, Y. Ding, N. Daskalakis, L. Jeuken, M. Povey, D.W. York, Mechanistic investigation into
antibacterial behavior of suspensions of ZnO nanoparticles against E. coli, J. Nanoparticle Res. 12 (2010) 1625-1636.
https://doi.org/10.1007/s11051-009-9711-1.
[2] S.L.C. Hsu, R.T. Wu, Synthesis of contamination-free silver nanoparticle suspensions for micro-interconnects,
Mater. Lett. 61(2007)3719-3722 https://doi.org/10.1016/j.matlet.2006.12.040.
[3] A. Keshavarz, A. Salabat, An efficient strategy in microemulsion systems to prepare mono- and bimetallic
platinum-rhenium reforming nanocatalyst with remarkable catalytic performance, Chem. select. 4 (2019) 6094-6100.
https://doi.org/10.1002/slct.201900376.
[4] S. Soleimani, A. Salabat, Effect of various factors on the Pt nanoparticle size produced in a microemulsion system,
Colloid J. 77(2015)207-212. https://doi.org/10.7868/S0023291215020172.
[5] S. Soleimani, A. Salabat, Rico F. Tabor, Effect of surfactant type on platinum nanoparticles size of the synthesized
Pt/α-Al2O3 catalyst by microemulsion method, J. Colloid Interface Sci. 426 (2014) 287-292.
https://doi.org/10.1016/j.jcis.2014.03.033.
[6] R.L. Johnston, Metal nanoparticles and nanoalloys, In Frontiers Nanosci. 3 (2012) 1-42
https://doi.org/10.1016/B978-0-08-096357-0.00006-6.
[7] S. Mohanty, S. Mishra, P. Jena, B. Jacob, B. Sarkar, A. Sonawane, An investigation on the antibacterial, cytotoxic,
and antibiofilm efficacy of starch-stabilized silver nanoparticles, Nanomed., Nanotech. Biol. Med. 8 (2012) 916-924.
https://doi.org/10.1016/j.nano.2011.11.007.
[8] C.P. Toumey, Reading Feynman into nanotechnology: A text for a new science, Techné: Research in Philosophy
and Technology. 12(2008)133-168 https://doi.org/10.5840/techne20081231.
[9] Q. Li, S. Mahendra, D.Y. Lyon, L. Brunet, M.V. Liga, D. Li, P.J. Alvarez, Antimicrobial nanomaterials for water
disinfection and microbial control: potential applications and implications, Water Res. 42 (2008) 4591-4602.
https://doi.org/10.1016/j.watres.2008.08.015.
[10] E. Hoseinzadeh, M.R. Samarghandi, M.Y. Alikhani, G. Asgari, G. Roshanaei, Effect of Zinc Oxide (ZnO)
nanoparticles on death kinetic of gram-negative and positive bacterium, J. Babol University Med. Sci. 14 (2012) 13-
19. https://doi.org/10.1080/19443994.2013.810356.
[11] K.Y. Yoon, J.H. Byeon, J.H. Park, J. Hwang, Susceptibility constants of Escherichia coli and Bacillus subtilis to
silver and copper nanoparticles, Sci. Total Environ. 373 (2007) 572-575. doi: 10.1016/j.scitotenv.2006.11.007.
[12] D. Lin, B. Xing, Phytotoxicity of nanoparticles: inhibition of seed germination and root growth, Environ
Pollution. 150 (2007) 243-250. https://doi.org/10.1016/j.envpol.2007.01.016.
[13] H. Kotrange, A. Najda, A. Bains, R. Gruszecki, P. Chawla, M.M. Tosif, Metal and Metal Oxide Nanoparticle as
a Novel Antibiotic Carrier for the Direct Delivery of Antibiotics, Int. J. Mol. Sci. 22 (2021)9596.
https://doi.org/10.3390/ijms22179596.
Colloid & Nanoscience Journal Original Article
Colloid Nanosci. J. 2(3) (2024) 390-396.
[14] Z. Huang, X. Zhang, Z. Yao, Y. Han, J. Ye, Y. Zhang, L. Chen, M. Shen, T. Zhou, Thymol-Decorated Gold
Nanoparticles for Curing Clinical Infections Caused by Bacteria Resistant to Last-Resort Antibiotics, 8 (2023) 54922,
https://doi.org/10.1128/msphere.00549-22.
[15] C. Singh, A.K. Mehata, V. Priya, A.K. Malik A. Setia, M.N. Suseela, L. Vikas, P. Gokul, Samridhi, S.K. Singh,
M.S. Muthu, Bimetallic Au-Ag Nanoparticles: Advanced Nanotechnology for Tackling Antimicrobial Resistance,
Molecules. 27 (2022)7059. https://doi.org/10.3390/molecules27207059.
[16] S. Martel, Method and system for controlling micro-objects or micro-particles. U.S. Patent Application No.
11/145(2006)007.